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. 2014 Dec 8;144(1):116–126. doi: 10.1111/imm.12356

Figure 2.

Figure 2

Antibody titres against various carriers following conjugation to mannosamine–biotin adduct (MBA). Mice were immunized twice, at 2-week intervals, with native or haptenated gp100, BSA or ovalbumin (OVA). Anti-carrier or anti-hapten antibody titre was determined as the reciprocal value of the twofold serial dilution end-point (twice the negative control OD value) of the serum as tested by ELISA (n = 4 to 6). Bar values represent mean antibody titre levels (±SD).*P < 0.05 and **P < 0.01 for the differences between anti-carrier antibody titre levels following immunization with native protein or MBA-conjugated carrier.