Figure 4.

Localization of haptenated protein in macrophage cellular compartment. Confocal microscopy analysis of antigen-presenting cells. RAW cells were seeded on eight-well Lab-Tek plates and incubated with 50 μg BSA conjugated to mannosamine–biotin (BSA–MBA) or BSA–biotin for 3 hr at 37°. After washing, fixation and permeabilization steps, intracellular staining for lysosome was performed with anti-LAMP1 (green) and carrier staining with avidin (red). Original magnification, 63 × Oil. Bars, 10 μm.