Figure 6.

T regulatory (Treg) cell population by flow cytometry analysis. Splenocytes were isolated and stained for CD4⁺ and CD25⁺ followed by intracellular staining for FoxP3⁺. CD25⁺, FoxP3⁺ cell frequencies out of CD4⁺ within the indicated gates are shown. Mice were immunized with horse serum (hs) IgG (i), hsIgG–biotin (ii), hsIgG– mannosamine–biotin (MBA) (iii), and saline (iv) (n = 6) and data represent two independent experiments (a). Bar values represent FoxP3⁺ percentage mean of gated CD4⁺ (±SD) (b). **P < 0.01 for the differences between hsIgG–MBA- and hsIgG-, hsIgG–biotin- or saline-immunized mice.