Table 2.
Antibody titres against hapten conjugated to self-protein or microparticles
| Anti-hapten antibody titre |
||
|---|---|---|
| Immunizing antigen | Mean | SD |
| hsIgG–MBA | 7780 | 902 |
| Mouse IgG–MBA | 2809 | 825 |
| Microparticles–MBA | < 32a | |
| Unconjugated MBA | < 32a | |
Mice were immunized twice with horse IgG conjugated to mannosamine–biotin (hsIgG–MBA), mouse IgG–MBA or microparticles–MBA. Anti-hapten antibody titre was determined as the reciprocal value of the twofold serial dilution end-point (twice the negative control OD value) of the serum as tested by ELISA (n = 4 to 6). Titres < 32 are equal to non-immune serum.
a
P < 0.05 for the differences between anti-MBA antibody titre levels following immunization with MBA-conjugated proteins and other treatments.