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. 2014 Dec 8;144(1):127–138. doi: 10.1111/imm.12357

Figure 6.

Figure 6

Dll1 and Dll4 expression is dependent upon interferon-β (IFN-β). (a) Human monocyte-derived macrophages (hMDM) were stimulated with rIFN-β (500 Units/ml) for 24 hr or vehicle-treated (ctrl). Dll1 and Dll4 mRNA expression was quantified by real-time PCR and normalized to that of GAPDH in each sample. (b) Protein levels of Dll1 and Dll4 were examined by Western blot. (c, d) Macrophages were infected with dengue virus serotype 2 (DENV2) (multiplicity of infection 4) for 36 hr in the absence or presence of the neutralizing antibody of IFN-β for 24 hr. Dll1 and Dll4 mRNA and protein expression was examined. Data are shown as mean ± SD of at least three independent experiments; *P < 0·05; **P < 0·01.