Figure 5.

High doses of oxazolone and IL-1β induce LC migration independently of TNF-α. Mice (BALB/c) received topical application of human LF (0·5 μg [a, c] or 5 μg [b]) in aqueous cream (or aqueous cream alone) to the dorsum of both ears. After 2 hr, mice received a topical application of 0·5% or 3% oxazolone (Ox, [a, b]) or intradermal injection of 50 ng or 300 ng IL-β (c) at the same site. Naïve control mice remained untreated. After a further 4 hr epidermal sheets were prepared and stained for LC as described previously and LC frequency expressed as mean LC/mm² per group ± SEM (each graph represents one individual experiment, n = 5–6 ears per group). The significance of differences between treated groups and naïve controls was analyzed by a one-way anova followed by Tukey's post-test (*P < 0·05, **P < 0·01, ***P < 0·001).