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. 2014 Dec 8;144(1):149–157. doi: 10.1111/imm.12358

Figure 3.

Figure 3

Increased antigen-specific T-cell proliferation in response to intranasal (i.n.) peptide delivery in B6.C3–/– mice. 2 × 106 CFSE-labelled Marilyn CD90.1 CD4+ CD25− T cells were injected intravenously into female recipient B6 wild-type (WT) or B6.C3–/– mice (three or four animals per group). Mice received i.n. three doses of the HY-AbDby peptide. Splenocytes were recovered 4, 5 and 6 days after the first peptide dose and proliferation of Marilyn T cells (gated as CD4+, CD90.1+) was analysed by flow cytometry. (a) Experimental schedule. (b) Representative data of gated Marilyn T-cell proliferation. (c) Percentage of Marilyn T cells with less than five divisions. (d) Foxp3 expression [mean fluorescence intensity (MFI)] in Marilyn T cells (left panel) and percentage of Foxp3+ Marilyn T cells (right panel). Results from control mice receiving PBS are indicated by open columns; those from peptide-treated mice are shown by filled columns. Data are shown as mean ± SEM. Statistical analysis by unpaired t-test; P-values as indicated; ns, non-significant.