Skip to main content
. 2014 Oct 29;15(12):1268–1277. doi: 10.15252/embr.201438850

Figure 1.

Figure 1

Cezanne is a regulator of HIF-1α transcriptional activity

A Depletion of Cezanne decreased HRE-luciferase activity in hypoxia. Data were normalised to hypoxia-treated HeLa cells transfected with non-targeting (NT) siRNAs.

B, C Knockdown of Cezanne significantly reduced Cezanne mRNA (B) and protein levels (C) relative to the NT control.

D RT–PCR analysis of endogenous HIF-1α target genes in U2OS cells depleted of Cezanne and subjected to hypoxia. Data are shown as fold change when compared to NT control.

E Cezanne-depleted cells show decreased LC3B-II and increased cleaved PARP levels.

F Quantification of apoptotic cells using annexin V staining. Loss of Cezanne reduced cell viability in hypoxia.

Data information: All experiments were performed three times; bar graphs represent the mean plus standard deviation of these independent experiments. P-values were calculated using Student's t-test (**P < 0.01, ***P < 0.001).