Figure 3.
Characterization of gut-associated lymphoid tissue (GALT) in RBP-JIEC-KO mice
A Peyer's patch (PP) tissues were analyzed for lymphoid follicle formation. Asterisks indicate lymphoid follicles. Data are representative of three independent experiments. Scale bars represent 1 mm.
B Surface area, the number of PPs, follicles per PP, and total number of PP lymphocytes were quantified. Values are presented as the mean ± standard deviation (n = 3–4). **P < 0.01, as calculated with the Student's t-test. n.s.: not significantly different.
C Immunofluorescence staining of the PP tissues from RBP-JIEC-KO mice and control littermates was performed using monoclonal antibodies against CD3ε (red) and B220 (green) and polyclonal antibodies against CCL21 or CXCL13 (green). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue). Data are representative of three independent experiments. Scale bars represent 0.2 mm.
D, E Whole-mount staining of small intestines from 10- to 14-week-old RBP-JIEC-KO and control mice was performed using a monoclonal antibody against B220. Representative macroscopic views of the serosal side of stained small intestines from RBP-JIEC-KO and control mice are shown (D). Scale bars represent 2 mm. The numbers of immature and mature ILFs in the small intestine were quantified (E). Values are presented as the mean ± standard deviation (n = 3). *P < 0.05, as calculated with the Student's t-test. n.s.: not significantly different.
Source data are available online for this figure.