Figure 4.
Compartmentalization of lymphoid follicles during the development of Peyer's patches (PPs) was impaired due to the abnormal expansion of goblet cells in RBP-JIEC-KO mice
A, B Whole-mount staining of the small intestines was performed using an anti-VCAM1 monoclonal antibody to detect PP anlagen and maturing PPs with lymphoid follicle compartmentalization at embryonic day 17.5 (E17.5) (A) and the neonatal stage (B), respectively.
C PP tissue sections were immunostained using polyclonal antibodies against CCL20 followed by Alcian blue.
D Whole-mount immunofluorescence staining of follicle-associated epithelium (FAE) from RBP-JIEC-KO mice was performed using polyclonal antibodies against CCL20 (green), Muc-2 (red), and F-actin (blue). Arrowheads represent goblet cells.
E Whole-mount specimens of the small intestine were immunostained using an anti-VCAM1 monoclonal antibody (left); tissue sections were then prepared from these specimens and stained with Alcian blue to detect goblet cells (right).
F The number of follicles per PP in 2-week-old mice was quantified. Values are presented as the mean ± standard deviation (n = 3–4). *P < 0.05, **P < 0.01, as calculated with one-way analysis of variance. n.s.: not significantly different.
Data information: Scale bars represent 200 (A, B, E left panel), 100 (C, D left panel, E right panel), or 20 (D center and right panels) μm. Data are representative of two (A, B, D, E) or three (C) independent experiments with similar results.
Source data are available online for this figure.