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. 2014 Dec 12;9(12):e115144. doi: 10.1371/journal.pone.0115144

Figure 4. Oxygen consumption rates differentially altered upon drug perturbation in sensitive compared to insensitive cells.

Figure 4

(A) NCI-H358 epithelial and mesenchymal lines or (B) NCI-H1703 (BPTES sensitive) and NCI-H1563 (BPTES insensitive) cells were treated with DMSO or 8 µM BPTES and oxygen consumption rate (OCR) was monitored over a 20 hr treatment. Data normalized to OCR at time zero (100%) and presented as mean values +/−SEM. P = 0.024 comparing changes in oxygen consumption with BPTES in the epithelial vs mesenchymal line; P = 0.00017 comparing NCI-H1703 and NCI-H1563 cells. (C) OCR following treatment with the indicated drugs to perturb mitochondrial respiration. Data normalized to OCR measurement prior to oligomycin treatment. (D) Addition of pyruvate to media restores the impaired FCCP response in the mesenchymal line. Average values +/−SEM indicated. Results representative of 2–3 independent experiments.