. Author manuscript; available in PMC: 2015 May 1.

Published in final edited form as: Cancer Discov. 2014 Sep 15;4(11):1342–1353. doi: 10.1158/2159-8290.CD-14-0622

Table 1.

Genomic status of STAG2, CDKN2A, and TP53 in Ewing sarcoma cell lines

Cell line	STAG2^a	CDKN2A^a	TP53^a
EW-3	p.R216*	wt	wt
EW-22	p.T463_L464fs	wt^b	p.R175H
EW-23	p.R807fs	wt	p.R273C
MHH-ES1	p.Q735fs	wt	p.S215del
MIC	p.R216*	wt	p.E285K
ORS	p.D625fs	wt	p.C176F
POE	p.F667fs	wt	p.L194R
SK-ES-1	p.Q735*	wt	C176F
SK-NM-C	p.M1_R546Del	wt	p.M1_T125Del
A673	wt	del(1a,1b,2,3)	p.A119fs
EW-1	wt	del(1a,1b,2,3)	p.R273C
EW-7	wt	del(1a)^b	wt
EW-16	wt	del(1a,1b,2,3)	p.K120fs
STA-ET-1	wt	del(1a,1b,2,3)	wt
TC-71	wt	del(1b,2,3)	p.R213*
STA-ET-3	wt	het^c	wt
EW-18	wt	wt	p.C176F
RD-ES	wt	wt	p.R273C
STA-ET-8	wt	wt	p.P152T

wt, wild-type.

STAG2 and TP53 mutations are annotated with respect to reference sequences NM_001042749 and NM_000546. For CDKN2A, numbers indicate the corresponding homozygous deleted exons (del) at this locus (exon 1a is specific for CDKNA2^INK4A, exon 1b is specific for CDKNA2^ARF, exons 2 and 3 are common to both).

Indicates a G->A polymorphism identified in EW-7 and EW-22 cell lines (rs3731249).

The STA-ET-3 cell line has a C to T heterozygous mutation (het) at position chr9:21,971,120 (hgu19), leading to nonsense (p.R80* for p16^INK4 based on NM_000077) and missense (p.P94L for p14^ARF based on NM_058195) mutations.