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. 2014 Oct 3;1(11):851–866. doi: 10.1002/acn3.118

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© 2014 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of acute GABA_BR1 KO on the expression CaSR and GABA_BR1 and apoptosis in cultured hippocampal neurons. (A and B) Immunoblots of lysates from cultured floxed-GABA_BR1 hippocampal neurons, which had been infected with Ad-Cont (lane 1) or Ad-Cre (lane 2) adenoviruses for 3 days, were performed for the expression of (A) GABA_BR1 and (B) CaSR. (C) TUNEL staining indicates that the Ad-Cre-infected hippocampal neuron cultures show a profound increase in the number of apoptotic cells depicted by brown DAB staining (green arrows). Cells are counterstained with hematoxylin. GABA_BR1, γ-aminobutyric acid receptor 1; KO, knockout; CaSR, Ca²⁺-sensing receptor; TUNEL, transferase dUTP nick end-labeling; DAB, 3,3′-diaminobenzidine.