Table 2.

Transcription ratios of target genes, chosen for confirmation of RNA-seq analysis during growth with vegetables and in M9-glucose medium obtained. Transcription of mRNA was determined by quantitative reverse transcription-PCR. Fold change was determined using software REST which calculates whether genes are significantly (P < 0.05) upregulated (Up) or downregulated (Down). Fold changes without Up or Down behind numbers show no significant differences in expression between vegetables and M9-glucose medium

Target gene	Sprouts 24 h	Sprouts 48 h	Sprouts 48 h RNA-seq	Lamb's lettuce 24 h	Spinach 24 h	Lettuce 24 h
csgB	232.18 Up	873.47 Up	1520.48 Up	2750.99 Up	3154.95 Up	5289.82 Up
glnK	0.07 Down	0.54	0.38 Down	0.69	0.14 Down	1.62
glpT	3.30 Up	3.52	1.13	2.04 Up	16.24 Up	24.36 Up
hutH	1.64	6.02 Up	5.06 Up	9.15 Up	23.35 Up	11.40 Up
nuoI	0.45 Down	2.06	1.09	1.97 Up	4.66 Up	15.50 Up
rcsA	0.80	3.84 Up	1.26	2.28 Up	1.87 Up	33.09 Up
sbp	41.62	30.27	16.12 Up	29.17 Up	0.59	118.95 Up