Table 3.
Primers designed for analysis of transcription ratios for target genes of S. Weltevreden 2007-60-3289-1 used for qRT-PCR
| Primera | Locus tag | Sequence (5′→3′) | Product size (bp) |
|---|---|---|---|
| csgB_F | SENTW_2110 | TAATCAGGCGGCCATTATTGG | 206 |
| csgB_R | TATTACCGTAAGCGCTTTGCG | ||
| hutH_F | SENTW_0770 | TTGAGGGCACAGGAGTTATTTGC | 194 |
| hutH_R | ACAGTGGTGATGTGATTCAGC | ||
| glpT_F | SENTW_2411 | TTAACGACTGGAAAGCGGCG | 178 |
| glpT_R | TTCGCAGTCAGCTCTTCTTCC | ||
| rcsA_F | SENTW_1101 | AACCTGACTCGCTGGATACC | 149 |
| rcsA_R | AATCTGAATGGTTCCCTGACC | ||
| sbp_F | SENTW_4153 | TTACGATGTGGACGCTATTGC | 175 |
| sbp_R | GTAATCACCGACACACCGGG | ||
| nuoI F | SENTW_2443 | TTACCGTGGTCGTATCGTGC | 219 |
| nuoI R | AACTGAATCGCCGTGGTCGG | ||
| glnK F | SENTW_0448 | GGGAGGCGCTTTCTTCCATT | 172 |
| glnK_R | ATCACCTCTTCCAGTTGGTCG | ||
| rpoD_F* | SENTW_3348 | ACATGGGTATTCAGGTAATGGAAGA | 61 |
| rpoD_R* | CGGTGCTGGTGGTATTTTCA | ||
| gmk F* | SENTW_3842 | TTGGCAGGGAGGCGTTT | 62 |
| gmk R* | GCGCGAAGTGCCGTAGTAAT |
a
Primers which were developed by (Botteldoorn et al., 2006) are indicated with an asterisk.