Fig 2.

In oxo-C14-HSL-pretreated barley plants, papillae formation is associated with expression of Peroxidase 7 and Pathogenesis Related 1. Sterile-grown barley cv. Golden Promise plants were pretreated with oxo-C14-HSL for 3 days prior to inoculation with Blumeria graminis f. sp. hordei.A. Formation of papillae in oxo-C14-HSL-pretreated and control plants on sites of attempted penetration by B. graminis. Image was taken 48 h after inoculation (hai) with B. graminis.B–C. Relative expression of HvPRX7 (B) and HvPR1 (C) in control and oxo-C14-HSL-pretreated plants assessed in hai as indicated. Expression values were normalized to the expression of HvUBQ60 and 0 hai time point. *P ≤ 0.05 in Student's t-test. Experiment was repeated three times.