Fig. 3.

STP1 regulation by Glc is mediated by an HXK1-independent signalling pathway. STP1 expression was analysed in wild-type plants and in the sugar signalling mutants gin2, abi4, and abi5. Samples were obtained from 12-day-old plants grown for 2 days in sugar starvation (–C) conditions and then transferred to –C, 150mM Glc, or Mtl media for 6h. Ten micrograms of total RNA was used for northern analysis and was hybridized with the STP1 probe. An initial control, which was measured before the treatments (T0), is included. The rRNA from the methylene blue-stained membranes is shown as a loading control. The membrane shown is representative of two biologically independent experiments.