Fig. 6.

Sugar regulates STP1 expression at a transcriptional level. Total RNA was isolated from 12-day-old plants from independent homozygous transgenic lines containing pSTP1-2.4::GUS. Prior to the treatment, the plants were grown for 2 days in media depleted of sugar (–) or supplemented with 150mM Glc (+) and then transferred to media without (–) or with 150mM Glc (+) for 6h. Each lane contains 10 µg of total RNA, and the blot was hybridized with the STP1 and GUS probes. The rRNA of the methylene blue-stained membrane is shown as a loading control. Membranes shown here are representative of three biologically independent experiments.