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. 2014 Oct 18;66(1):85–97. doi: 10.1093/jxb/eru401

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Localization and activity of Golgi lumenal localized human-codon optimized Renilla luciferase (hRluc). Rat sialyltransferase transmembrane domain (ST) fused to hRluc was used to target hRluc to the Golgi apparatus. (A) ST–hRluc-YFP co-localized with the cis-Golgi marker α-mannosidase-CFP. Scale bar=20 µm. (B) ST–hRluc and hRluc activities in transiently expressing N. benthamiana crude leaf protein extracts. The silencing suppressor p19 was co-expressed with ST–hRluc and hRluc constructs and alone is used as a negative control for background luminescence. Error bars represent 95% confidence interval, n=3. Log₁₀(RLU); relative luminescence units transformed to Log₁₀. (This figure is available in colour at JXB online.)