Fig. 5.

Application of the Rluc-PCA to test PPIs among XyG biosynthetic enzymes. Three independent experiments, each consisting of three biological replicates (pools) were made (see materials and methods). Generated results are shown as heat map of Log₁₀ values of RLU where dark grey denotes samples with all experiments being significantly higher than the background level, whereas light grey denotes samples with two out of three experiments being significantly higher than the background level and white denotes Log₁₀ values of RLU of the background p19 infiltrated control in a complementation assay. Statistical analysis was performed on the averages derived from three independent experiments. Plants were co-transfected with Agrobacteria carrying vectors containing silencing suppressor p19, GOI–hRluc[F1], and GOI–hRluc[F2]. Error represents 95% confidence interval, n=3.