Table 2.
Comparison of the results obtained by Rluc-PCA, the split-ubiquitin assay (Split-Ub), and bimolecular fluorescence complementation (BiFC)/co-immunoprecipitation (Co-IP) (Chou et al., 2012)0, 1, and 2, indicate no PPI, a PPI with low confidence, and a PPI with high confidence, respectively. nt indicates not tested or not testable owing to non-functional or non-expressed proteins. POI, protein of interest.
| Combination | Rluc-PCA | Split-Ub | BiFC/Co-IP | |
|---|---|---|---|---|
| POI 1 | POI 2 | |||
| XXT1 | XXT1 | 0 | Nt | 0 |
| XXT2 | 2 | Nt | 2 | |
| XXT5 | 1 | Nt | 1 | |
| MUR3 | 1 | 0 | nt | |
| FUT1 | 0 | 0 | nt | |
| CSLC4 | nt | Nt | 0 | |
| XXT2 | XXT2 | 0 | Nt | 2 |
| XXT5 | 1 | 0 | 2 | |
| MUR3 | 1 | 2 | nt | |
| FUT1 | 1 | 0 | nt | |
| CSLC4 | nt | 0 | 0 | |
| XXT5 | XXT5 | 0 | Nt | 1 |
| MUR3 | 2 | 0 | nt | |
| FUT1 | 2 | 0 | nt | |
| CSLC4 | nt | Nt | 2 | |
| MUR3 | MUR3 | 2 | 2 | nt |
| FUT1 | 2 | 1 | nt | |
| CSLC4 | nt | 0 | nt | |
| FUT1 | FUT1 | 2 | 0 | nt |
| CSLC4 | nt | 0 | nt | |
| CSLC4 | CSLC4 | nt | Nt | 2 |