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. 2014 Oct 8;66(1):113–124. doi: 10.1093/jxb/eru403

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — HSP90.7 constructs and screening of transgenic plants that express HSP90.7 or HSP90.7^Δ22. (A) Schematic diagrams of HSP90.7 and HSP90.7^Δ22 coding sequence constructs in binary vectors used for plant transformation. The charged fragment D488–G509 in HSP90.7 is deleted in HSP90.7^Δ22. The C-terminal FLAG tag and ER-retention sequences are shown above. (B, C) Immunoblotting of transgenic plants expressing FLAG-tagged wild-type HSP90.7 (B) and HSP90.7^Δ22 (C). Total proteins were prepared from 10-d-old T2 seedlings, resolved by 8% SDS-PAGE, and immunoblotted with anti-FLAG and anti-HSP90.7 antibodies.