Fig. 1.

Neighbor-joining phylogenetic tree of UGT80- and UGT713-related proteins. Arabidopsis thaliana UGT80A2, UGT80B1, and UGT713B1 (white boxes) are conserved among vascular plants (green shading and circles) and mosses (blue shading and squares). UGT80 enzymes also share similarity with predicted proteins from various filamentous fungi (red shading and triangles), whereas UGT713 is limited to embryophytes. Saccharomyces cerevisiae UGT51A1, a functional sterol glucosyltransferase enzyme (red shading and triangle), was included for comparison. Asterisks denote sequences that were not assigned UGT names due to incomplete sequence or lack of EST support. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown at the branches. Evolutionary distances were computed using the Poisson correction method and analysis was conducted in MEGA5.2.2 (Tamura et al., 2011). The cladogram is rooted with respect to Arabidopsis thaliana At2g19880, a predicted glucosylceramide synthase (GCS) (yellow diamond).