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. 2014 Oct 14;66(1):189–201. doi: 10.1093/jxb/eru410

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — ASG composition in wild-type and ugt80 and ugt713 mutant seeds. For the samples in Fig. 4, ASG compounds were detected by ESI-MS/MS. (A) Total ASG, and (B) 16:0, (C) 18:2, (D) 18:1, (E) 18:0, and (F) 20:0 ASG are shown for wild type (WT), ugt80A2, ugt80B1, ugt713B1, each corresponding double mutant and the triple mutant. The usg80A2,B1 double mutant shows significant decreases in ASG levels. For the single mutants the most striking reductions in ASG were observed in ugt80B1 for 18:3 ASG: (G) total 18:3 ASGs, and (H) 18:3 sitosteryl, (I) 18:3 campesteryl, and (J) 18:3 stigmasteryl ASGs are indicated for all mutant combinations. 18:3 brassicasteryl and cholesteryl ASGs are given in Supplementary Table S5. Error bars denote standard deviations (n=5), and significant differences from WT are marked by an asterisk (two-tailed t-test, P≤0.05).