Fig. 1.

Identification of Arabidopsis mutants gln1;1, gln1;2, and gln1;1:gln1;2. (A) RT–PCR analysis of Gln1;1 and Gln1;2 expression in the roots of the wild-type (Wt), gln1;1, gln1;2, and gln1;1:gln1;2 plants. UBQ2 was used as a reference gene for equal amounts of cDNA in different samples. Each sample contained a pool of roots from five individual plants. (B) Western blot analysis of GS1 and GS2 contents in shoots of Wt, gln1;1, gln1;2, and gln1;1:gln1;2 plants. The upper band corresponds to GS2, and the lower band corresponds to GS1. The same amount of crude protein was loaded in each lane, as described in the Materials and methods. Each sample contained a pool of shoots from five individual plants.