Berry development in Physalis species. (A) Flower development. The developmental stage of the mature flower (F) was set as d 0, and the floral buds at 9 (B1), 6 (B2), and 3 (B3) d before anthesis (DBA) are presented. (B) Fruit development. The developing berries of 5, 10, 15, 20, 25, 30, and 50 DPA are presented. The top images are intact fruits, and the bottom images represent the berries in which the inflated calyx was removed. The developmental stages in P. philadelphica were similarly defined for harvesting of tissues. Bars, 1cm. (C) Cell number variation. (D) Cell size variation. Quantification was performed according to median transverse section of the developing carpel and fruit from the floral buds (B1, B2, and B3), mature flower (F) and the developing berries of 5 and 50 DPA (see Materials and Methods for details). Five median transverse sections for each stage sample were analysed. The cell size of 150 cells for each tissue on each section was measured. The cell number for each section was counted. The mean and standard deviation are presented. The details are presented in Tables S3 and S4.