Fig. 6.

Complementation experiment of a loss-of-function OsCPS1 mutant by ectopic expression of OsCPS2. T₁ seeds were obtained from several lines of T₀ plants, transgenic heterozygous oscps1-1 (NE3024) plants into which OsCPS1p::OsCPS2 cDNA (Fig. 5) was introduced. (A) Results of genotyping and images of T₁ seedlings of line 2, the knockout line (no. 3), complemented line (no. 15), and the segregated wild-type line (no. 4). Tos-CPS1, bands from Tos17-inserted OsCPS1 genome DNA (Tos17-F and CPS1-WT-R in Supplementary Fig. S1 available at JXB online); WT-CPS1, bands from wild-type OsCPS1 genomic DNA (CPS1-WT-F and CPS1-WT-R in Supplementary Fig. S1); endoCPS2, bands from native OsCPS2 genomic DNA (with intron); transCPS2, bands from transgene OsCPS2 cDNA (no intron). The sense primer (CPS2-QPCR-F; Supplementary Table S) and antisense primer (CPS2-QPCR-R; Supplementary Table S1) for OsCPS2 genotyping were designed from the nucleotide sequences of exons 10 and 11, respectively. (B) Images of adult T₁ rice plants of line 1, the segregated wild-type line (no. 8), and the complemented line (no. 2).