Fig. 3.

Glucose, fructose, and sucrose contents on day 92 in the leaves of A. alpina plants grown continuously at 20 °C (warm, grey) or transferred to 5 °C on day 63 (cold, white). Locations are presented in order of increasing altitude of origin (A–H) Dependent on the accession (see Materials and methods) leaf 14 or leaf 16 was classified as mature (open bars) and leaf 12 or leaf 14 as old (hashed bars). Data are means of leaves from three to five plants+SEM. Asterisks indicate statistically significant differences between the temperature treatments or age groups (ANOVA; *P≤ 0.05; **P≤ 0.01); different letters indicate differences between the groups (Tukey’s test). n.a.=not available.