Fig. 3.

Expression of early auxin genes and several PIN genes in 14-day-old light-grown seedlings. Seedlings were either treated with 10 µM IAA in (A) (grey bars: 10min, black bars: 30min) or mock in (B). qRT-PCRs were from three biological replicates with three technical replicates for each gene. Statistical analysis was performed as described by Livak and Schmittgen (2001) and verified using the method of Pfaffl et al. (2002). At t=0min fold expression was set as 1 for the wt in (B). Asterisks indicate significant difference to the wt (* P<0.05; ** P<0.01).