Table 1.
Sample type | Amount (mg) | Analysis | Number of samples (n) |
---|---|---|---|
Immature ovary tissue, pooled from several females | 20 | Microarray and qPCR | 3 |
Early vitellogenic follicles, pooled from several females | 20 | Microarray and qPCR | 3 |
Late vitellogenic follicles, pooled from several females | 20 | Microarray and qPCR | 3 |
Ovulated eggs, pooled from one femalea | 20 | Microarray | 3 |
Blastula embryos, pooled from one femalea | 20 | Microarray and qPCR | 3 |
Gastrula embryos, pooled from one femalea | 20 | Microarray and qPCR | 3 |
Samples from Kleppe et al. (2013). Each sample is a pool of eggs/embryos from one female. Three samples at the same developmental stage represent sibling pools from three different females.
Type and amount of each sample, downstream analysis and number of samples in each analysis are shown.