Fig 2.

IFNα does not perturb HCV particle composition or density. HCV (SA13/JFH)-infected Huh-7.5 cells were treated with IFNα (1,000 IU/mL) for 1 hour, washed thoroughly, and the extracellular media collected for a further 1 hour. HCV Core and E2 expression in extracellular purified virus and cell lysates was assessed by western blot and densitometric imaging (expressed in arbitrary light units, ALU) (A) while HCV RNA was quantified by qRT-PCR (B). The lipid density of the treated and untreated virus was assessed using 10-60% Iodixanol gradient centrifugation: the graphs depict the infectivity, HCV RNA, and density of each fraction from IFN-treated or untreated cells (C).