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. 2014 Nov 4;117(6):1549–1563. doi: 10.1111/jam.12662

Figure 1.

Figure 1

Phylogenetic tree of CYP105 homologues in Streptomyces. Amino acid sequences for CYP105s in Streptomyces spp. were aligned (Sievers et al. 2013) and a phylogenetic tree generated. The tree was obtained in Mega6 using the neighbour-joining method (Tamura et al. 2013). The values shown are confidence percentages based on a bootstrap value of 500 repetitions. The scale bar (top left) indicates a genetic change of 0·2. Only CYP105s assigned to a subfamily by Nelson (http://drnelson.uthsc.edu/CytochromeP450.html) are included, so the list should not be considered exhaustive. Also included were examples of CYP105 enzymes from other actinomycete genera, MoxA from Nonomuraea recticatena and a CYP105 from Kutzneria. The final proteins included as comparators were CYP170A1, a well-characterized P450 from Streptomyces coelicolor, and CYP2R1, a human P450 with low level amino acid homology to CYP105 enzymes.