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. 2014 Nov 30;14:280. doi: 10.1186/s12866-014-0280-9

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© Burda et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Nitrosoguanidine mutagenesis identifies additional regulators of sigS expression. (A) The SH1000 sigS-lacZ fusion strain was subjected to MNNG mutagenesis. Two resulting clones were selected for further analysis during growth in TSB at 37°C. HKM15 and HKM16 were grown along side the parent strain, SH1000, with samples collected at 5 hours. β-Galactosidase activity was measured using 4-MUG as a substrate to determine sigS expression levels. Assays were performed on duplicate samples and the values averaged. The results presented are from three independent experiments. Error bars are shown as ± SEM. (B) qPCR for sigS expression was performed on SH1000 strains containing a mutation in either lacR or kdpD. Error bars are shown as ± SEM. Significance was determined using a Student t test; *indicates a p value of <0.05.