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. 2014 Dec 4;14:247. doi: 10.1186/s12862-014-0247-3

Table 1.

Summary of analysed Caullerya mesnili samples from seven lakes in the Czech Republic

DNA Lake Year TCS-type No. of ITS
Extraction C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 Sequences
20 pooled hosts/lake sample a
Brno 2004 6 0 0 10 0 11 0 0 0 0 27
Brno 2005 2 0 0 12 1 2 0 14 0 1 32
Římovc 2004 3 0 0 19 0 7 0 1 0 0 30
Římov 2005 2 0 0 22 0 4 0 0 1 0 29
Římov 2008 0 0 0 15 0 4 0 10 0 0 29
Římov 2009 3 0 0 15 0 8 0 4 0 0 30
Stanovice 2004 3 0 0 18 0 5 0 1 0 0 27
Stanovice 2005 3 0 0 11 1 10 0 0 0 0 25
Trnávka 2005 8 0 0 12 0 7 0 0 0 0 27
Vírc 2004 9 0 0 2 0 17 0 0 0 0 28
Vír 2005 3 0 0 15 0 6 0 1 0 0 25
Vír 2009 4 0 0 5 0 17 0 0 0 0 26
Vranov 2004 2 0 0 10 0 14 0 3 0 0 29
Vranov 2008 3 0 0 22 0 7 0 0 0 0 32
Vranov 2009 3 0 0 12 0 18 0 0 0 0 33
Želivkac 2004 1 0 0 8 2 6 0 11 0 0 28
∑ 55 0 0 208 4 143 0 45 1 1 457
10 single hosts/lake sample b
Římovc 2004 19 3 0 66 0 20 0 9 0 0 117
Vírc 2004 34 1 1 20 0 56 0 0 0 0 112
Želivkac 2004 10 0 0 26 7 22 1 43 0 0 109
∑ 63 4 1 112 7 98 1 52 0 0 338

Specifically, the number of ITS sequences obtained from C. mesnili parasite DNA, as well as their assignment to representative sequence variants (TCS-types) by statistical parsimony analysis (C1-C10, the labels are consistent with [38]) are provided. Parasite DNA was either extracted from 10 individual Daphnia hosts per sample or 20 pooled Daphnia hosts per sample. Table modified after Giessler and Wolinska [40].

aParasite data from Giessler and Wolinska [40].

bParasite data from Wolinska et al. [38].

cParasite DNA from these samples was analysed using both approaches (i.e. 10 single hosts and 20 pooled hosts were genotyped, respectively).