Table 1.
Summary of analysed Caullerya mesnili samples from seven lakes in the Czech Republic
| DNA | Lake | Year | TCS-type | No. of ITS | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Extraction | C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 | C9 | C10 | Sequences | ||
| 20 pooled hosts/lake sample a | |||||||||||||
| Brno | 2004 | 6 | 0 | 0 | 10 | 0 | 11 | 0 | 0 | 0 | 0 | 27 | |
| Brno | 2005 | 2 | 0 | 0 | 12 | 1 | 2 | 0 | 14 | 0 | 1 | 32 | |
| Římovc | 2004 | 3 | 0 | 0 | 19 | 0 | 7 | 0 | 1 | 0 | 0 | 30 | |
| Římov | 2005 | 2 | 0 | 0 | 22 | 0 | 4 | 0 | 0 | 1 | 0 | 29 | |
| Římov | 2008 | 0 | 0 | 0 | 15 | 0 | 4 | 0 | 10 | 0 | 0 | 29 | |
| Římov | 2009 | 3 | 0 | 0 | 15 | 0 | 8 | 0 | 4 | 0 | 0 | 30 | |
| Stanovice | 2004 | 3 | 0 | 0 | 18 | 0 | 5 | 0 | 1 | 0 | 0 | 27 | |
| Stanovice | 2005 | 3 | 0 | 0 | 11 | 1 | 10 | 0 | 0 | 0 | 0 | 25 | |
| Trnávka | 2005 | 8 | 0 | 0 | 12 | 0 | 7 | 0 | 0 | 0 | 0 | 27 | |
| Vírc | 2004 | 9 | 0 | 0 | 2 | 0 | 17 | 0 | 0 | 0 | 0 | 28 | |
| Vír | 2005 | 3 | 0 | 0 | 15 | 0 | 6 | 0 | 1 | 0 | 0 | 25 | |
| Vír | 2009 | 4 | 0 | 0 | 5 | 0 | 17 | 0 | 0 | 0 | 0 | 26 | |
| Vranov | 2004 | 2 | 0 | 0 | 10 | 0 | 14 | 0 | 3 | 0 | 0 | 29 | |
| Vranov | 2008 | 3 | 0 | 0 | 22 | 0 | 7 | 0 | 0 | 0 | 0 | 32 | |
| Vranov | 2009 | 3 | 0 | 0 | 12 | 0 | 18 | 0 | 0 | 0 | 0 | 33 | |
| Želivkac | 2004 | 1 | 0 | 0 | 8 | 2 | 6 | 0 | 11 | 0 | 0 | 28 | |
| ∑ | 55 | 0 | 0 | 208 | 4 | 143 | 0 | 45 | 1 | 1 | 457 | ||
| 10 single hosts/lake sample b | |||||||||||||
| Římovc | 2004 | 19 | 3 | 0 | 66 | 0 | 20 | 0 | 9 | 0 | 0 | 117 | |
| Vírc | 2004 | 34 | 1 | 1 | 20 | 0 | 56 | 0 | 0 | 0 | 0 | 112 | |
| Želivkac | 2004 | 10 | 0 | 0 | 26 | 7 | 22 | 1 | 43 | 0 | 0 | 109 | |
| ∑ | 63 | 4 | 1 | 112 | 7 | 98 | 1 | 52 | 0 | 0 | 338 | ||
Specifically, the number of ITS sequences obtained from C. mesnili parasite DNA, as well as their assignment to representative sequence variants (TCS-types) by statistical parsimony analysis (C1-C10, the labels are consistent with [38]) are provided. Parasite DNA was either extracted from 10 individual Daphnia hosts per sample or 20 pooled Daphnia hosts per sample. Table modified after Giessler and Wolinska [40].
aParasite data from Giessler and Wolinska [40].
bParasite data from Wolinska et al. [38].
cParasite DNA from these samples was analysed using both approaches (i.e. 10 single hosts and 20 pooled hosts were genotyped, respectively).