Table 1.

PCR primers for human pathogens associated w. Ixodes scapularis ticks, DOD Human Tick Test Kit Program

Pathogen	Year	Initial screen	Reference	Reconfirmation a	Reference
Anaplasma phagocytophilum
	1997-2003	16S rRNA	[9]	16S rRNA	[9]
	2004-2012	groEL	[10]	16S rRNA	[9]
Borrelia burgdorferi
	1997-2003	p66	[14]	fla	[15]
	2004-2012	ospA	[16]	fla	[17]
Babesia microti
	2002-2008	ss-rDNA	[11]	18 s rRNA	[12]
	2009-2012	ss-rDNA	[13]	18 s rRNA	[12]
Ehrlichia muris -like agent (EML)
	2007-2012	groEL	[10]	sequenced

^aReconfirmation of samples positive in the initial screen.