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. 2014 Nov 30;5:27. doi: 10.1186/s13100-014-0027-z

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© Ross et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Tn5 / IS50 structure and gene expression. (A) The structure of Tn5 is shown along with transcription units within IS50-Right. There are two distinct promoters defined by -35/-10 regions that control transposase (black) and inhibitor (blue) expression. T1 is the transposase transcript and T2 is the inhibitor transcript. The Shine-Dalgarno sequence of T1 is also shown. Expression of T1 but not T2 is regulated by DAM methylation at two GATC sequences (red) and potentially LexA binding (dotted line defines a putative LexA binding site). Mutations in the dam sites used in this work are shown. kan ^R, ble ^R and str ^R are kanamycin, bleomycin and streptomycin resistance genes, respectively. (B) Post-transcriptional regulation by Hfq. Hfq (blue hexamer) is shown catalyzing the pairing of an sRNA with an mRNA. The sRNA can be either cis or trans encoded relative to its target mRNA. In both cases the sRNA is shown pairing to the translation initiation region of the mRNA (asterisks) and would block translation.