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. 2014 Nov 30;5:27. doi: 10.1186/s13100-014-0027-z

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© Ross et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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IS50 transposase mRNA half-life analysis. Strains TM338 (wt) and TM618 (hfq ⁻) were transformed with IS50 transposase encoding plasmid pDH533 and total RNA was isolated either before or after the addition of rifampicin (at the indicated time points). Transposase RNA was detected as described in Figure 5. The bands were quantified (ImageQuant) and T1 normalized to un-extended primer before plotting the proportion of RNA remaining after rifampicin addition (time zero = 1.0). The data was fit to a one-phase exponential decay curve by non-linear regression (Prism) to determine the half-life (t_1/2). The data shown is a compilation from two independent experiments.