Figure 9.
Impact of SgrS expression on Tn5 transposition. Transposition of a chromosomal copy of Tn5 was measured in DBH179 using the ‘mating out’ assay. DBH179 containing one of the indicated plasmids was grown overnight in M9 glucose and then subcultured in either M9 glucose or M9 glucose plus glycerol before mating with the recipient strain and plating on selective media as described in Figure 2. IPTG was added to the subculture (to 0.1 mM) to induce SgrS expression, except where indicated (-IPTG). The average transposition frequency for the ‘no SgrS’ control was 5.52 × 10−5 events per mL of mating mix. All other transposition frequencies were normalized to this value. Mean and standard error values of duplicate experiments, each of which included at least five replicates for each experimental group, are shown.