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. 2014 Dec 9;14:100. doi: 10.1186/s12896-014-0100-1

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© Nainys et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Titration curves of swine serum samples. Either positive or negative for PCV2 antibodies swine serum samples were titrated by the newly developed indirect IgG PCV2 Cap VLP-based ELISA using recombinant PCV2 Cap VLPs at a concentration of 0.5 μg/ml (solid lines) and control antigen (HaPyV-VP1) at a concentration of 2 μg/ml (dotted line). Sera were tested in serial duplicate twofold dilutions ranging from 1:100 to 1:3200. The results are expressed as the OD₄₅₀ and correspond to the average of the values obtained in at least two different analyses.