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. 2014 Dec 3;7:165. doi: 10.1186/s13068-014-0165-z

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© Clarkson et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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24-h substrate and end product analysis. T. pseudethanolicus was grown at 65°C with 40 mM glucose and increasing concentrations of (A) furfural or (B) 5-HMF for 24 h. Culture supernatants were analyzed for glucose and end product formation by HPLC. Glucose consumption was determined by comparison with an uninoculated control. Error bars are the standard deviation of three replicate cultures.