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. 2014 Dec 3;7:165. doi: 10.1186/s13068-014-0165-z

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© Clarkson et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Overexpression of Teth39_1597 and Teth39_1598 in Escherichia coli . Teth39_1597 (48.4 kDa) and Teth39_1598 (42.1 kDa) were cloned from T. pseudethanolicus into pET-30a and overexpressed from a T7 promoter after induction with IPTG. Overexpression was determined in whole cell extract by Coomassie-stained SDS-PAGE.