Table 4.
Specific activity (μmol/min/mg protein) of putative T. pseudethanolicus 39E aldehyde reductases
| Vector control | Teth39 BdhA | Teth39 AKR | |
|---|---|---|---|
| Furfural | 0.33 ± 0.13 | 4.97 ± 0.17 | 0.27 ± 0.04 |
| 5-Hydroxymethylfurfural | 0.44 ± 0.16 | 10.06 ± 0.80 | 0.39 ± 0.07 |
| Acetaldehyde | 0.02 ± 0.15 | 4.54 ± 0.76 | 0.02 ± 0.01 |
| Butyraldehyde | 0.09 ± 0.05 | 14.58 ± 3.57 | 0.33 ± 0.04 |
| Isobutyraldehyde | −0.10 ± 0.21 | 0.63 ± 0.29 | 0.23 ± 0.05 |
Whole cell lysate with pET-30a only (vector control) or expressing Teth39 BdhA (Teth39_1597) or Teth39 AKR (Teth39_1598) was assayed aerobically at 60°C using 0.2 mM NAD(P)H as the electron donor and 20 mM substrate. NAD(P)H oxidation activity was measured via loss of absorbance at 340 nm. Values in boldface are statistically significant. No activity was detected with either Teth39 BdhA or Teth39 AKR above vector control using NADH.