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. 2014 Dec 1;7:863. doi: 10.1186/1756-0500-7-863

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© Jumat et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phylogenetic tree of the NS gene segment of Singaporean specimens isolated from 2004 to 2009. The NS sequences of these specimens were aligned together with the NS gene segments of B/Lee/40, B/Yamagata/16/88, B/Victoria/2/87, the vaccine strains from 2004–2013 (Table 1) and the NA gene segments of Singaporean strains isolated in 1991, 1994 and 1998. Owing to the lack of NS gene sequences of recent strains from SEA, the NS alignment also included strains from the United States, Egypt and New Zealand which were isolated post 2000. The 3 different clusters of NS1 were labelled Clades I, II and III.