Table 10.
Amino acid substitution of the NS1-NLS AND ISG15 binding site
| NS1 nuclear localisation sequence | ISG15 binding site | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 46 | 56 | 34 | 97 | 101 | |||||||||||
| I | B Lee 40 | D | R | L | H | R | L | N | R | K | L | E | F | I | E |
| DSO 090114 2004 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| DSO 090124 2004 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| DSO 090131 2004 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| DSO 090133 2004 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| DSO 090134 2004 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| DSO 090136 2004 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| DSO 090138 2004 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| II | B Yamagata 16 1988 | . | . | . | N | . | . | . | K | . | . | . | L | V | . |
| B Victoria 02 1987 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| B Singapore 04 1991 | . | . | . | N | . | . | . | K | . | . | . | L | V | . | |
| B Singapore 11 1994 | . | . | . | N | . | . | . | K | . | . | . | L | V | ||
| III | DSO 090117 2004 | . | . | . | N | . | . | . | K | . | . | . | L | . | . |
| DSO 050143 2005 | . | . | . | N | . | . | . | K | . | . | . | L | . | G | |
| DSO 050540 2005 | . | . | . | N | . | . | . | K | . | . | . | L | . | G | |
| Rest of DSO Specimens | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| B Singapore 22 1998 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| B Singapore 31 1998 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| B Singapore 35 1998 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| Vacc04S B Hong Kong 330 2001 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| Vacc0405N 05S B Jilin 20 2003 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| Vacc0405N 05S B Shanghai 361 2002 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| Vacc0608NS B Malaysia 2506 2004 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| Vacc0506N B Jiangsu 10 2003 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| Vacc0809NS B Florida 4 2006 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| Vacc0912NS B Brisbane 60 2008 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
| Vacc1213N B Wisconsin 01 2010 | . | . | . | N | . | . | . | K | . | . | . | L | . | . | |
Amino acids 46–56 correspond to the NLS of the NS1 protein. I: Specimens clustering in cluster I of the NS phylogenetic tree. II: Specimens clustering in cluster II of the NS phylogenetic tree. III: Specimens clustering in cluster III of the NS phylogenetic tree. Rest of DSO Specimens: rest of specimens sequenced in this study not individually listed in this table. Identical amino acids are marked by a dot (.).