Table 9.

Amino acid substitution of the NB protein

	Transmembrane domain								Ecto domain
	21	22	24	29	30	31	34	35	43	48	67	92
B Yamagata 16 1988	V	I	T	F	T	V	T	V	F	N	P	L
Rest of DSO Specimens	I	.	.	.	I	.	.	I	P	Y	.	.
DSO 070214 2006	I	.	I	.	I	I	.	I	L	.	.	.
DSO 050091 2006	I	.	I	.	I	I	.	I	L	.	.	.
DSO 020147 2007	I	.	I	.	I	I	.	I	L	.	.	.
DSO 020132 2007	I	.	I	.	I	I	.	I	L	.	.	.
DSO 020114 2007	I	.	I	.	I	I	.	I	L	.	.	.
DSO 020113 2007	I	.	I	.	I	I	.	I	L	.	.	.
DSO 050528 2005	I	.	.	.	I	.	.	I	P	Y	.	F
DSO 0003 2009	I	.	.	.	I	.	.	I	.	Y	S	I
DSO 0005 2009	I	.	.	.	I	.	.	I	.	Y	S	I
DSO 0070 2009	I	.	.	.	I	.	.	I	.	Y	S	I
DSO 0100 2009	I	.	.	.	I	.	.	I	.	Y	S	I
B Singapore 04 1991	.	T	.	L	I	.	.	.	L	Y	.	.
B Singapore 11 1994	A	.	.	.	.	.	.	.	.	Y	.	.
B Singapore 22 1998	.	.	.	.	.	.	I	I	.	Y	.	.
B Singapore 31 1998	.	.	.	.	.	.	I	I	.	Y	.	.
B Singapore 35 1991	.	.	.	.	.	.	.	I	.	Y	.	.
B Singapore 222 1979	.	.	.	.	.	.	.	.	.	Y	.	.
Vacc04S B Hong Kong 330 2001	.	.	.	L	.	.	.	.	.	.	.	F
Vacc0405N 05S B Jilin 20 2003	.	.	.	.	.	.	.	I	.	.	.	.
Vacc0405N 05S B Shanghai 361 2002	.	.	.	.	.	.	.	I	.	.	.	.
Vacc0608NS B Malaysia 2506 2004	I	.	.	.	I	.	.	I	P	Y	.	.
Vacc0506N B Jiangsu 10 2003	.	.	.	.	.	.	.	I	.	.	.	.
Vacc0809NS B Florida 4 2006	.	.	.	.	I	.	.	I	.	.	.	.
Vacc0912NS B Brisbane 60 2008	.	.	.	.	I	I	.	I	L	.	.	.
Vacc1213N B Wisconsin 01 2010	.	.	.	.	I	.	.	I	.	.	.	.
B Lee 40	I	I	T	L	T	V	I	V	F	N	P	L
B Victoria 02 1987	V	I	T	L	I	V	T	V	F	N	P	L

Amino acid 21–35 correspond to the putative transmembrane domain of the NB protein while positions 43–92 are located on the ecto domain of the protein. Specimens listed under B Yamagata 16 88 belong to the Yamagata. Rest of DSO Specimens: rest of specimens sequenced in this study not individually listed in this table. Identical amino acids are marked by a dot (.).