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. 2014 Dec 2;14:898. doi: 10.1186/1471-2407-14-898

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© Burghoff et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Low ecto-AMPase activity in B16-F10 cells. AMPase activity was measured by the dephosphorylation of etheno-AMP to etheno-adenosine in cultured B16-F10 cells using HPLC. CD73 and alkaline phosphatase (AP) activity were assessed using the AP inhibitor levamisole (50 μM) and the CD73 inhibitor AOPCP (50 μM) respectively. Data are shown as mean ± SEM (n = 3).