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Negative ion electrospray UHPLC-MS/MS with selected reaction monitoring (SRM) chromatograms of prenylated hop phenols in human urine from a single subject obtained A) before enzymatic deconjugation; and B) after deconjugation using β-glucuronidase and sulfatase. Note the large increase in the levels of prenyl phenols after enzymatic deconjugation, indicating that conjugates (especially glucuronides) were the major circulating forms of these compounds.
