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. 2014 Dec 2;14:153. doi: 10.1186/1471-2415-14-153

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© McElnea et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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LC cell auto-fluorescence reflecting cellular lipopfuscin content. The lipofuscin auto-fluorescence emitted by approximately 10,000 cells in the yellow-green range of the spectrum (563-607 nm, FL-1 channel) was quantified by flow cytometry. Glaucomatous LC cells demonstrated increased whole cell auto-fluorescence relative to normal LC cells (83.6 ± 45.1 MFI vs. 41.0 ± 3.9 MFI, p = 0.02, n = 3).