Fig. 1.

Interaction of p53 with USP11

(a) HEK293 cells were cotransfected with Flag-USP11 and HA-p53. Cells were collected and lysed 24 h after transfection. Cell lysates were subjected to immunoprecipitation with anti-Flag antibody and immunoblotted with anti-HA antibody. (b) U2OS cells were treated with or without 20 μmol/L etoposide for 24 h. Cells were harvested and lysed. We adjusted the p53 to the same level and then performed the co-immunoprecipitation assay