Fig. 4.

Role of USP11 on p53 induction in response to DNA damage

(a) U2OS cells stably expressing USP11 shRNA or control cells were treated with 20 μmol/L etoposide for 12 h. Cells were harvested and cell lysates were subjected to immunoblotting analysis with indicated antibodies. (b) Total RNAs were extracted, reverse-transcribed, and analyzed by quantitative real-time PCR for the indicated genes (p21, bax, and puma). Results are shown as mean±SD of triplicate experiments and normalized to the control